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Department of Chemistry, Graduate School of Science, Kyoto University
 

Masayuki Endo (Associate Professor)

Field of Expertise:
Bioorganic Chemistry, Chemical Biology, DNA Nanotechnology
Research Interests:
  1. Development of single-molecule imaging and analysis using DNA nanospace
  2. Development of DNA molecular devices and system using DNA nanostructures
  3. Applications for cell functional regulation using DNA nanodevices
Papers:
  1. K. Abe, Y. Hirose, H. Eki, K. Takeda, T. Bando, M. Endo, H. Sugiyama, X-ray crystal structure of a cyclic-PIP–DNA complex in the reverse-binding orientation. J. Am. Chem. Soc. 142, 10544-10549 (2020).
  2. S. Jonchhe, S. Pandey, D. Karna, P. Pokhrel, Y. Cui, S. Mishra, H. Sugiyama, M. Endo, H. Mao, DNA duplex is Weakened in Nanoconfinement. J. Am. Chem. Soc. 142, 10042-10049 (2020).
  3. I. Mela, P. Vallejo-Ramirez, S. Makarchuk, G. Christie, D. Bailey, R. Henderson, H. Sugiyama, M. Endo, C. Kaminski, DNA nanostructures as a tool for targeted antimicrobial delivery. Angew. Chem. Int. Ed. 59, 12698-12702 (2020).
  4. X. Xing, S. Sato, K. Hidaka, H. Sugiyama, M. Endo, Direct Observation and Analysis of TET-mediated Oxidation Processes in a DNA Origami Nanochip. Nucleic Acids Res. 48, 4041-4051 (2020).
  5. G. Raghavan, K. Hidaka, H. Sugiyama, M. Endo, Direct observation and analysis of the dynamics of the photoresponsive transcription factor GAL4. Angew. Chem. Int. Ed. 58, 7626-7630 (2019).
  6. D. Ishikawa, Y. Suzuki, C. Kurokawa, M. Ohara, M. Tsuchiya, M. Morita, M. Yanagisawa, M. Endo, R. Kawano, M. Takinoue, DNA origami nanoplate-based emulsion with designed nanopore function. Angew. Chem. Int. Ed. 58, 15299-15303 (2019).
  7. S. Jonchhe, S. Pandey, T. Emura, K. Hidaka, M. A Hossain, P Shrestha, H. Sugiyama, M. Endo, H. Mao, Decreased water activity in nanoconfinement contributes to the folding of G-quadruplex and i-motif structures. Proc. Natl. Acad. Sci. USA, 115, 9539-9544 (2018).
  8. T. Masubuchi, M. Endo, R. Iizuka, A. Iguchi, D. H. Yoon, T. Sekiguchi, H. Qi, R. Iinuma, Y. Miyazono, S. Shoji, T. Funatsu, H. Sugiyama, Y. Harada, T. Ueda, H. Tadakuma, Construction of integrated gene logic-chip. Nature Nanotechnology, 13, 933-940 (2018).
  9. Y. Suzuki, H. Sugiyama, M. Endo, Complexing DNA origami frameworks through sequential self-assembly based on shape-complementarity docking. Angew. Chem. Int. Ed. 57, 7061-7065 (2018).
  10. T. Kurokawa, S. Kiyonaka, E. Nakata, M. Endo, S. Koyama, E. Mori, N. H. Tran, H. Dinh, Y. Suzuki, K. Hidaka, M. Kawata, C. Sato, H. Sugiyama, T. Morii, Y. Mori, DNA Origami Scaffolds as Templates for Functional Tetrameric Kir3 K+ Channels. Angew. Chem. Int. Ed. 57, 2586-2591 (2018).
  11. A. Lee, M. Endo, J. Hobbs, C. Wälti, Direct Single-Molecule Observation of Mode and Geometry of RecA-Mediated Homology Search. ACS Nano, 12, 272-278 (2018).
  12. E. M. Willner, Y. Kamada, Y. Suzuki, T. Emura, K. Hidaka, H. Dietz, H. Sugiyama, M. Endo, Single-Molecule Observation of The Photoregulated Conformational Dynamics of DNA Origami Nanoscissors. Angew. Chem. Int. Ed. 56, 15324-15328 (2017).
  13. Y. Kobayashi, O. Misumi, M. Odahara, K. Ishibashi, M. Hirono, K. Hidaka, M. Endo, H. Sugiyama, H. Iwasaki, T. Kuroiwa, T. Shikanai, Y. Nishimura, Holliday-junction resolvases mediate chloroplast nucleoid segregation. Science, 356, 631-634 (2017).
  14. P. Shrestha, S. Jonchhe, T. Emura, K. Hidaka, M. Endo, H. Sugiyama, H. Mao, Confined Space Facilitates G-quadruplex Formation. Nature Nanotechnology, 12, 582−588 (2017).
  15. T. Shibata, Y. Fujita, H. Ohno, Y. Suzuki, K. Hayashi, K. R. Komatsu, S. Kawasaki, K. Hidaka, S. Yonehara, H. Sugiyama, M. Endo, H. Saito, Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate. Nature Communications, 8, 540 (2017).
  16. M. H. Räz, K. Hidaka, S. J. Sturla, H. Sugiyama, M. Endo, Torsional Constraints of DNA Substrates Impact Cas9 Cleavage. J. Am. Chem. Soc. 138, 13842-13845 (2016).
  17. A. Kuzyk, Y. Yang, X. Duan, S. Stoll, A. O. Govorov, H. Sugiyama, M. Endo, N. Liu, A light-driven 3D plasmonic nanosystem that translates molecular motion into reversible chiroptical function. Nature Communications, 7, 10591 (2016).
  18. Y. Yang, M. Goetzfried, K. Hidaka, M. You, W. Tan, H. Sugiyama, M. Endo, Direct visualization of walking motions of a photo-controlled DNA nanomachine on the DNA nanostructure. Nano Lett. 15, 6672-6676 (2015).
  19. Y. Suzuki, M. Endo, H. Sugiyama, Lipid bilayer-supported two-dimensional self-assembly of DNA origami nanostructures. Nature Communications, 6, 8052 (2015).
  20. M. Endo, Y. Suzuki, T. Emura, K. Hidaka, F. Wang, I. Willner, H. Sugiyama, Single-Molecule Visualization of the Activity of Zn2+-Dependent DNAzyme. Angew. Chem. Int. Ed. 54, 10550-10554 (2015).
  21. M. Endo, X. Xing, X. Zhou, T. Emura, K. Hidaka, B. Tuesuwan, H. Sugiyama, Single-Molecule Manipulation of the Duplex Formation and Dissociation at the G-Quadruplex/i-Motif Site in the DNA Nanostructure. ACS Nano, 9, 9922-9929 (2015).
  22. M. Endo, H. Sugiyama, Single-Molecule Imaging of Dynamic Motions of Biomolecules in DNA Origami Nanostructures Using High-Speed Atomic Force Microscopy. Acc. Chem. Res. 47, 1645-1653 (2014).
  23. A. Rajendran, M. Endo, H. Sugiyama, State-of-the-Art High-Speed Atomic Force Microscopy for the Investigation of Single-Molecular Dynamics of Proteins. Chem. Rev. 114, 1493-1520 (2014).
  24. Y. Suzuki, M. Endo, Y. Katsuda, K. Ou, K. Hidaka, H. Sugiyama, DNA origami based visualization system for studying site-specific recombination events. J. Am. Chem. Soc. 136, 211-218 (2014).
  25. Y. Suzuki, M. Endo, Y. Yang, H. Sugiyama, Dynamic Assembly/Disassembly Processes of Photoresponsive DNA Origami Nanostructures Directly Visualized on a Lipid Membrane Surface. J. Am. Chem. Soc. 136, 1714-1717 (2014).
  26. A. Rajendran, M. Endo, K. Hidaka, H. Sugiyama, Direct and single-molecule visualization of the solution-state structure of G-hairpin and G-triplex intermediates. Angew. Chem. Int. Ed. 53, 4107−4112 (2014).
  27. M. Endo, S. Yamamoto, T. Emura, K. Hidaka, N. Morone, J. E. Heuser, H. Sugiyama, Helical DNA Origami Tubular Structures with Various Sizes and Arrangements. Angew. Chem. Int. Ed. 53, 7484-7490 (2014).
  28. D. Koirala, P. Shrestha, T. Emura, K. Hidaka, S. Mandal, M. Endo, H. Sugiyama, H. Mao, Single-Molecule Mechanochemical Sensing Using DNA Origami Nanostructures. Angew. Chem. Int. Ed. 53, 8137-8141 (2014).
  29. A. Rajendran, M. Endo, K. Hidaka, H. Sugiyama,Direct and real-time observation of rotary movement of a DNA nanomechanical device. J. Am. Chem. Soc. 135, 1117-1123 (2013).
  30. A. Rajendran, M. Endo, K. Hidaka, P. L. T. Tran, J.-L. Mergny, R. J. Gorelick, H. Sugiyama, HIV-1 Nucleocapsid Proteins as Molecular Chaperones for Tetramolecular Antiparallel G-Quadruplex Formation. J. Am. Chem. Soc. 135, 18575-18585 (2013).
  31. S. F. J. Wickham, J. Bath, Y. Katsuda, M. Endo, K. Hidaka, H. Sugiyama, A. J. Turberfield, A DNA-based molecular motor that can navigate a network of tracks. Nature Nanotechnology, 7, 169-173 (2012).
  32. M. Endo, R. Miyazaki, T. Emura, K. Hidaka, H. Sugiyama, Transcription Regulation System Mediated by Mechanical Operation of DNA Nanostructure. J. Am. Chem. Soc. 134, 2852-2855 (2012).
  33. T. Yoshidome, M. Endo, G. Kashiwazaki, K. Hidaka, T. Bando, H. Sugiyama, Sequence-Selective Single-Molecule Alkylation with a Pyrrole-Imidazole Polyamide Visualized in a DNA Nanoscaffold. J. Am. Chem. Soc. 134, 4654-4660 (2012).
  34. Y. Yang, M. Endo, K. Hidaka, H. Sugiyama, Photo-Controllable DNA Origami Nanostructures Assembling into Predesigned Multiorientational Patterns. J. Am. Chem. Soc. 134, 20645-20653 (2012).
  35. M. Endo, Y. Yang, Y. Suzuki, K. Hidaka, H. Sugiyama, Single-Molecule Visualization of the Hybridization and Dissociation of Photoresponsive Oligonucleotides and Their Reversible Switching Behavior in a DNA Nanostructure. Angew. Chem. Int. Ed. 51, 10518-10522 (2012).
  36. M. Endo, K. Tatsumi, K. Terushima, Y. Katsuda, K. Hidaka, Y. Harada, H. Sugiyama, Direct Visualization of the Movement of a Single T7 RNA Polymerase and Transcription on a DNA Nanostructure. Angew. Chem. Int. Ed. 51, 8778-8782 (2012).
  37. A. Rajendran, M. Endo, H. Sugiyama, Single-Molecule Analysis Using DNA Origami. Angew. Chem. Int. Ed. 51, 874-890 (2012).
  38. S. F. J. Wickham, M. Endo, Y. Katsuda, K. Hidaka, J. Bath, H. Sugiyama, A. J. Turberfield, Direct observation of stepwise movement of a synthetic molecular transporter. Nature Nanotechnology, 6, 166-169 (2011).
  39. A. Rajendran, M. Endo, K. Hidaka, H. Sugiyama, Photo-Cross-Linking-Assisted Thermal Stability of DNA Origami Structures and Its Application for Higher-Temperature Self-Assembly. J. Am. Chem. Soc. 133, 14488-14491 (2011).
  40. M. Endo, Y. Katsuda, K. Hidaka, H. Sugiyama, A versatile DNA nanochip for direct analysis of DNA base-excision repair. Angew. Chem. Int. Ed. 49, 9412−9416 (2010).
  41. M. Endo, Y. Katsuda, K. Hidaka, H. Sugiyama, Regulation of DNA Methylation Using Different Tensions of Double Strands Constructed in a Defined DNA Nanostructure. J. Am. Chem. Soc. 132, 1592−1597 (2010).
  42. Y. Sannohe, M. Endo, Y. Katsuda, K. Hidaka, H. Sugiyama, Visualization of Dynamic Conformational Switching of the G-Quadruplex in a DNA Nanostructure. J. Am. Chem. Soc. 132, 16311−16313 (2010).
  43. M. Endo, K. Hidaka, T. Kato, K. Namba, H. Sugiyama, DNA Prism Structures Constructed by Folding of Multiple Rectangular Arms. J. Am. Chem. Soc. 131, 15570–15571 (2009).
Academic careers:
1992　BSc, Department of Synthetic Chemistry, Kyoto University
1997　Ph.D, Department of Chemistry and Biotechnology, The University of Tokyo
1997　Tokyo Medical and Dental University, JSPS postdoc
1998　Department of Chemistry and Chemical Biology, Harvard University, CRI postdoc
2000　RIKEN, SPDR postdoc
2001　Assistant Professor, Institute of Scientific and Industrial Research, Osaka University
2005　Associate Professor, Institute of Scientific and Industrial Research, Osaka University
2008　Associate Professor, Institute for Integrated Cell-Material Sciences, Kyoto University
2018　Associate Professor, Graduate School of Science, Kyoto University
Contact Information:
Room: Institute for Integrated Cell-Material Sciences Main Building
Phone: 81(Japan)-75-753-9765
E-mail: endo　＋　@kyoto-u.ac.jp
Link:
Bioarchitecture Laboratory Home Page

This page was last modified on 05 August 2020.
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