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■京化■京都大学理学部化学科・京都大学大学院理学研究科学専攻
　

藤井 紀子（教授）

専門分野
生化学、蛋白質科学、加齢科学
研究テーマ
  1. 放射線、紫外線照射による蛋白質への影響研究
  2. D-アミノ酸を指標とする加齢の科学
  3. 蛋白質中でのD-アミノ酸生成機構の研究
  4. 結合型D-アミノ酸分析の開発
代表的な論文
  1. Isomeric replacement of a single aspartic acid induces a marked　change in protein function: The Example of Ribonuclease A.
    Sakaue H, Kinouchi T, Fujii N, Takumi T and Fujii N. ACS Omega 2, 260−267 (2017).
  2. LC-MS/MS analysis of post-translational modifications including oxidation and deamidation of rat lens α- and β-crystallins induced by γ-irradiation.
    Kim I, Saito T, Fujii N, Kanamoto T and Fujii N. Amino Acids. 48, 2855-2866 (2016).
  3. New insight into the dynamical system of αB-crystallin oligomers.
    Takata T, Fujii N, Ishii K, Uchiyama S, Sato N, Oba Y, Wood K, Kato K, Fujii N & Sugiyama M. Scientific Reports 6, 29208 (2016).
  4. Isomerization of Asp residues plays an important role in αA-crystallin dissociation
    Takata, T and Fujii N. FEBS J. 83, 850-859 (2016).
  5. Isomerization of aspartyl residues in crystallins and its influence upon cataract
    Fujii N, Takata T, Fujii N and Aki K.Biochimica et Biophysica Acta 1860,183-191 (2016).
  6. Site specific oxidation of amino acid residues in rat lens γ-crystallin induced by low dose γ-irradiation.
    Kim I, Saito T, Fujii N, Kanamoto T, Chatake T, Fujii N.Biochem.Biophys.Res.Commu. 466, 622-628 (2015).
  7. Effect of Asp 96 isomerization on the properties of a lens αB-crystallin-derived short peptide.
    Takata T and Fujii N. J. Pharm. Biomed Anal. 116, 139-144 (2015)
  8. Interaction of αA-crystallin F71L mutant with wild type αA- and αB-crystallins by mammalian two hybrid assay.
    Ramkumar S, Thankappan B, Fujii N, Natarajaseenivasan K, Anbarasu K. Int J Biol Macromol. 76, 102-108, (2015). doi: 10.1016/j.ijbiomac.2015.02.025. May.
  9. Real-time heterogeneous protein-protein interaction between αA-crystallin N-terminal mutants and αB-crystallin using quartz crystal microbalance (QCM).
    Ramkumar S, Fujii N, Sakaue H, Fujii N, Thankappan B, Kumari RP, Natarajaseenivasan K, Anbarasu K.Amino Acids. 47, 1035-1043 (2015). doi: 10.1007/s00726-015-1935-z.
  10. Rapid Survey of Four Asp Isomers in Disease-Related Proteins by LC-MS combined with Commercial Enzyme.
    Maeda H, Takata T, Fujii N, Sakaue H, Nirasawa S, Takahashi S, Sasaki H and Fujii N. Anal. Chem 87, 561-568 (2015).
  11. αB- and βA3-crystallins containing D-Aspartic acids exist in a monomeric state.
    Sakaue H, Takata T, Fujii N, Sasaki, H and Fujii N. Biochimica et Biophysica Acta -Proteins and Proteomics, 1854, 1–9 (2015).
  12. In vitro Amyloidogenic Peptides of Galectin-7: Possible Mechanism of Amyloidogenesis of Primary Localized Cutaneous Amyloidosis.
    Ono K, Fujimoto E, Fujimoto N, Akiyama M, Satoh T, Maeda H, Fujii N, Tajima S. J Biol Chem. 289, 29195-29207 (2014.)
  13. Comparison of effect of gamma ray irradiation on wild-type and N-terminal mutants of αA-crystallin.
    Ramkumar S, Fujii N, Fujii N, Thankappan B, Sakaue H, Ingu K, Natarajaseenivasan K, Anbarasu K. Mol Vis. 20:1002-1016 (2014).
  14. Effects of carotenoids on damage of biological lipids induced by gamma irradiation.
    Saito T. and Fujii N. Radiat. Phys. Chem. 98, 57-63 (2014).
  15. Simultaneous ultraviolet-B induced photo-oxidation of　tryptophan/tyrosine and racemization of neighboring aspartyl residues in peptides.　
    Cai S, Fujii N, Saito T, and Fujii N. Free Radical Biology & Medicine. 65, 1037-1046 (2013).
  16. Kinetics of isomerization and inversion of aspartate 58 of αA-crystallin peptide mimics under physiological conditions.　
    Kenzo Aki, Norihiko Fujii and Noriko Fujii. PLoS One. 8 (3):e58515 doi:10.1371/journal.pone.0058515. (2013).
  17. A rapid comprehensive LC-MS based survey of the Asp isomers in crystallins from human cataract lenses.　
    Norihiko Fujii, Hiroakai Sakaue, Hiroshi Sasaki and Noriko Fujii. J Biol Chem. 287, 39992-40002 (2012).
  18. A P39R mutation at the N-terminal domain of human alpha B-crystallin regulates its oligomeric state and chaperone-like activity.
    Numoto N, Kita A, Fujii N, Miki K. Biochem Biophys Res Commun. 31, 601-606. (2012).
  19. Chronic exposure to low frequency noise at moderate levels causes impaired balance in mice.
    Tamura H, Ohgami N, Yajima I, Iida M, Ohgami K, Fujii N, Itabe H, Kusudo T, Yamashita H, Kato M.　 PLoS One. 7(6):e39807. Epub 2012 Jun 29.(2012).
  20. Co-localisation of advanced glycation end products and D-beta-aspartic acid-containing proteins in gelatinous drop-like corneal dystrophy.
    Kaji Y, Oshika T, Takazawa Y, Fukayama M, Fujii N.　Br J Ophthalmol.　96,1127-1131 (2012).
  21. Structural features of isomerizable aspartyl residues in human alpha-crystallins.
    Ken-ichi Shimizu, Akiko Kita, Noriko Fujii, and Kunio Miki. Molecular Vision, 18, 1823-1827(2012).
  22. Characterization of an antibody that recognizes peptides containing D-beta-aspartyl residues.
    Kenzo Aki, Norihiko Fujii, Takeshi Saito and Noriko Fujii . Molecular Vision, 18, 996-1003 (2012).
  23. Simultaneous stereoinversion and isomerization at the Asp-4 residue in βB2-crystallin from the aged human eye lenses.
    Norihiko Fujii, Takeshi Kawaguchi, Hiroshi Sasaki and Noriko Fujii. Biochemistry 50, 8628-8635 (2011).
  24. UV B-irradiation enhances the racemization and isomerizaiton of aspartyl residues and production of Nε-carboxymethyl lysine (CML) in keratin of skin.
    Yuhei Mori, Kenzo Aki, Katsunori Kuge, Natsuko Yamanaka, Shingo Tajima, Yuichi Kaji, Ryoji Nagai, Hanako Yoshii, Naoki Yamamoto, Norihiko Fujii, Masami Watanabe, Tadatoshi Kinouchi and Noriko Fujii. J. Chromatogr. B 879, 3303-3309 (2011)
  25. Influence of Lβ-, Dα-, and Dβ-Asp isomers of the Asp-76 residue on the properties of αA-crystallin 70-88 peptide.
    Noriko Fujii, Norihiko Fujii, Masashi Kida and Tadatoshi Kinouchi. Amino Acids 39, 1393-1399 (2010).
  26. Differentiation and Semiquantitative analysis of an isoaspartic acid in human alpha -crystallin by Post Source Decay on Curved Field Reflectron.
    Yuzo Yamazaki, Norihiko Fujii, Yutaka Sadakane and Noriko Fujii. Anal. Chem. 82, 6384−6394 (2010)
  27. The Protective Effects of Metallothionein I and II against Metal- or Ultraviolet Radiation-Induced Damages in Cultured Lens Epithelial Cells. Saito T, Tezuka T, Konno R. and Fujii N. Jpn J. Ophthalmol. 54, 486-493 (2010)
  28. Accumulation of D-β-Aspartic acid-containing proteins in age-related ocular diseases.
    Yuichi Kaji, Tetsuro Oshika, Yutaka Takazawa, Masashi Fukayama, Noriko Fujii. Chemistry and Biodiversity 7, 1364-1370 (2010).
  29. SANS and SAXS Observations of Abnormal Aggregation of alpha-Crystallin.
    Masaaki Sugiyama, Norihiko Fujii, Yukio Morimoto, Keiji Itoh, Kazuhiro Mori, Toshiharu Fukunaga, and Noriko Fujii. Chemistry and Biodiversity 7, 1380-1388 (2010).
  30. Influence of oxidative stress on D-aspartyl endopeptidase activity.
    Tadatoshi Kinouchi, Takahiko Shimizu, Takuji Shirasawa and Noriko Fujii. Chemistry and Biodiversity 7, 1398-1402 (2010)
  31. Structural consideration of mammalian D-aspartyl endopeptidase.
    Tadatoshi Kinouchi and Noriko Fujii. Chemistry and Biodiversity 7, 1403-1407 (2010)
  32. Oxidative Stress Indces the Formation of D-Aspartyl Residues in the Elastin Mimic Peptide.
    Katusnori Kuge, Keiko Kitamura, Koichi Nakaoji, Kazuhiko Hamada, Norihiko Fujii, Takeshi Saito, Noriko Fujii. Chemistry and Biodiversity 7, 1408-1412 (2010)
  33. Localization of D-β-Aspartyl Residue-Containing Proteins in Various Tissues.
    Motoie R, Fujii N,* Tsunoda S, Nagata K, Shimo-oka T, Kinouchi T, Fujii N, Saito T and Ono K. Int. J. Mol. Sci. 10, 1999-2009(2009).
  34. A Simple and Rapid Method for Measurement of (10)B-para-Boronophenylalanine in the Blood for Boron Neutron Capture Therapy Using Fluorescence Spectrophotometry.
    Kashino G, Fukutani S, Suzuki M, Liu Y, Nagata K, Masunaga S, Maruhashi A, Tanaka H, Sakurai Y, Kinashi Y, Fujii N, Ono K. Radiat Res (Tokyo). 50, 377-382 (2009).
  35. Tryptophanase-Catalyzed l-Tryptophan Synthesis from d-Serine in the Presence of Diammonium Hydrogen Phosphate.
    Shimada A, Ozaki H., Saito T. and Fujii N. Int. J. Mol. Sci. 10, 2578-2590 (2009).
  36. Immunohistochemical localization of D-β-aspartic acid-containing proteins in climatic droplet keratopathy.
    Kaji Y, Oshika T, Takazawa Y, Fukayama M, Fujii N. Br. J. Ophthalmol. 93, 977-979 (2009).
  37. Immunohistochemical localization of D-beta-aspartic acid in pinguecula.
    Kaji Y, Oshika T, Okamoto F, Fujii N. Br J Ophthalmol. 93, 974-976 (2009)
  38. Screening system for D-Asp-containing proteins using D-aspartyl endopeptidase and two-dimensional gel electrophoresis.
    Sakai-Kato K, Kinouchi T, Fujii N, Imai K, Utsunomiya-Tate N. Amino Acids 36, 125-129 (2009).
  39. Study of subunit interactions of alpha A- and alpha B- crystallins and the effects of gamma-irraditation on their interactions by surface plasmon resonance.
    Fujii N. Hisano T. and Fujii N. Biochim. Biophys. Acta 1784, 1507-1513 (2008).
  40. Differential rate constants of racemization of aspartyl and asparaginyl residues in human alpha A-crystallin mutants.
    Nakamura T., Sakai M., Sadakane Y., Haga T., Goto Y., Kinouchi T., Saito T, and Fujii N. Biochim. Biophys. Acta 1784, 1192-1199 (2008).
  41. Structural evolution of human recombinant alpha B-crystallin under UV irradiation.
    Sugiyama M., Fujii N., Morimoto Y., Kurabayashi S., Vigild, M., Sato T., Nakagawa T., Ito K., Mori K. and Fukunaga T. Biomacromolecules 9, 431-434 (2008).
  42. Localization of D-beta-aspartic acid-containing proteins in human eyes.
    Kaji Y, Oshika T, Takazawa Y, Fukayama M, Takata T, Fujii N. Invest. Ophthalmol. Vis. Sci. 48, 3923-3927 (2007)
  43. Conformation-dependent racemization of aspartyl residues in peptides.
    Kuge K., Brack A., and Fujii N. Chem. Eur. 13, 5617-5621 (2007)
  44. Shape of alpha-Crystallin Analyzed by Small-Angle Neutron Scattering.
    Masaaki Sugiyama M., Fujii N., Morimoto Y., Otomo T., Takata T., Misawa M, Annaka M., Mori K., Sato T., Kurabayashi S. and Fukunaga T. J. Appl. Crystal. 40, 200-204 (2007)
  45. Age related changes of alpha-crystallin aggregate in human lens.
    Fujii N, Shimmyo Y, Sakai M, Sadakane Y, Nakamura T, Kinouchi T, Morimoto Y, Goto Y and Lampi K. Amino Acids 32, 87-94 (2007)
  46. Isolation and characterization of mammalian D-aspartyl endopeptidase.
    Kinouchi T, Nishio H, Nishiuchi Y, Tsunemi M, Takada K, Hamamoto T, Kagawa Y, Fujii N. Amino Acids 32, 79-85 (2007).
  47. Differential susceptibility of alpha A- and alpha B-crystallin to gamma-ray irradiation.
    Fujii N., Nakamura T, Sadakane Y, Saito T. and Fujii N. Biochim. Biophys. Acta 1774, 345-350 (2007)
略歴・受賞
1976-1980　東京医科歯科大学大学院医学研究科博士課程
1980-1985　筑波大学文部技官
1987-1991　筑波大学化学系助手
1991-1994　武田薬品工業株式会社開拓第１研究所
1994-1997　科学技術振興事業団「さきがけ研究２１」「場と反応」領域研究者
1997-1998　工業技術院産業技術融合領域研究所特別技術補助職員
1998-2002　京都大学原子炉実験所・助教授
2002-　　　現職

1998年　第３回「日本女性科学者の会」奨励賞受賞
受賞名：「D-アミノ酸を指標とした老化の基礎研究」
2015年　第8回日本光医学・光生物学会　学会賞受賞
役員
1998-2002　生命の起源及び進化学会 "Viva Origino"　編集委員長
2002-現在　生命の起源及び進化学会・事務局長
2003-現在　US-JAPAN CCRG世話人
2004-現在　水晶体研究会世話人
2004-現在　日本光医学・光生物学会理事
2004-現在　D-アミノ酸研究会運営委員長
2006-現在　科学技術振興機構男女共同参画アドバイザリー委員会委員
2006-現在　日本女性科学者の会理事
2007-現在　日本白内障学会理事
連絡先
部屋番号　原子炉実験所219号室
電話番号　072-451-2496（直通）
ファクス番号　072-451-2630
電子メール　nfujii　＋　@rri.kyoto-u.ac.jp
リンク
放射線生命化学分科紹介（京化サイト内）
藤井研究室ホームページ

（最終更新日；2018年04月05日）
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